P P < 0.05). As denoted by the midpoint of the fermentation, all fermentation runs, with and without trub, at lower wort boil durations fermented faster than longer or higher wort-boiling treatments. The decline in wort fermentability was highest upon treatment at wort temperature and pressure levels of 121.1 °C and 3.0 atm. The amount of trub and the wort color formed after each heat treatment showed a gradual increase with heating duration. Free amino nitrogen levels of wort declined significantly with respect to both increase in wort boiling temperature and time intervals (